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FEATURES

Image Capture

• Acquire files directly from camera, scanner, disk, or CD-ROM

• Streamlined capture interface - New

• Get an optimized view with dynamic auto-range - New

• User-defined live scaling for low-light acquisition -New

• AutoSet feature takes the guesswork out of setting exposure parameters

• Semi-transparent live preview feature assists in tiling, stitching, and aligning images

• Save and change settings based on camera or application with a single click

• Capture multiple images after user-specified delay/acquisition time

• Acquire, create, and playback a sequence (stack) of images

• Apply filters or operations to individual frames or to entire sequence with sequencer playback option

• Includes capture support for 24-, 36-, and 48-bit color; 8-, 12-, and 16-bit gray scale input devices

Memory Management
 

Image Processing Tools 
 

• Stitch and tile images 

• Automatic alignment of image stacks to correct for stereo, rotational, and other types of shifts

• N-dimensional viewer lets you choose variables to display from image sets

• Create in-focus composite images from partially in-focus source image stacks with EDF (Extended Depth of Field)

• EDF Test Strips automatically tests all EDF options on your images with a single click

• Set Manager allows you to easily organize, extract, and navigate multiple image sets

• Dye Manager lets you apply dyes to gray scale images by choosing emission wavelength

• Local Zoom tool allows you to simultaneously view your entire image in one window, while viewing a more magnified area of that image in a second window

• Work with topographic maps for composite in-focus images

• Create composite in-focus images with maximum local contrast, maximum through depth contrast, maximum intensity and minimum intensity

• Create composite images from an infinite number of source gray scale images with the Color Composite Tool

• Use the Sequence Gallery to produce a montage image that allows for preview of all image analysis processes on image stacks

• Perform spatial and logical arithmetic, as well as image alignment operations

• Combine images with Image Overlay

• Create, print, or save a collage of several images with Mosaic Image

• Combine the results of two segmentation algorithms with Restricted Dilation Feature

• Create grids for intercept counting

• Use Image Registration tools for comparative analysis

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Image Enhancement Tools
 

• Edit image intensity level with Display Range

• Work with background subtraction and correction

• Manually or automatically enhance color and contrast using equalization, gamma correction, contouring, or thresholding

• Invert, reset, and apply contrast to images

• Reverse pixel values with automatic image inversion

Image Filtering
 

• Work with Filter dialogs that include descriptions and interactive preview windows

• Use Enhancement filters that include Low-Pass, Hi-Pass, Gaussian, Hi-Gaussian, Sharpen, Flatten, Median, Rank, Local Equalization, and Despeckle in user-definable kernel sizes

• Outline objects with various Edge Filter methods including Sobel, Roberts, Laplace, Variance, Phase, Horizontal, and Vertical

• Process objects with Morphological filters including Erode, Dilate, Open, Close, Top Hat, Well, Branch/End, Watershed, Thinning, Pruning, Distance, and Reduce in user-definable kernel sizes

• Work with Large Spectral Filters: Fast-acting, large kernel size (up to 4000 x 4000) filters that include Low-Pass, Hi-Pass, Band-Pass, and Edge filters

• Apply filters to individual frames or to entire sequence

• Customize kernel files for morphological and convolution filters

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Fast Fourier Transform (FFT)
 

• Create forward and inverse transforms

• Edit the spectrum directly including Low-Pass, High-Pass, unsharpen, spike cut, and spike boost

• Save and reload data

Fluorescense Lookup Tables and Overlays
 

• New feature to apply tint to gray scale images automatically

• Extract and merge multiple color channels (including RGB, HSI, HSV, and YIQ) simultaneously

• Use color information to separate objects from background

• Segment image based on the color channel histogram or color cube

• Set pre-defined and custom color spreads (ranges) with the Pseudocolor tool

3D Imaging Tools (Requires Open GL graphics card)

• Voxel-based 3D filters for use with 3D volumetric, time-lapse and other 3D image sets - New in 6.0

• Built-in Open GL viewer for interactive 3D volume rendering - New in 6.0

SharpStack Deconvolution Tools

• Built-in real-time 2D deconvolution - New in 6.2

• Built-in Nearest Neighbor and No-Neighbor deconvolution - New in 6.2

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Spatial and Intensity Calibration
 

• Calibration wizard helps you to calibrate an active image and create reference and system calibrations

• Use X and Y scale bars as a nondestructive overlay

• Work with intensity or spatial measurements

• Create and display spatial calibration markers

• Create and display system calibrations

• Use pre-defined spatial calibration units

• Save and recall all calibrations

• Calculate derived calibrations automatically

• Store information about your microscope in lens information files

Classify Objects
 

• Count and Size objects automatically

• Measure areas, perimeters, lengths, roundness, major and minor axes, aspects, angles, centroids, holes, population density - over 50 measurements

• Identify over 100,000 objects per frame (memory dependent) - New in 6.0

• Set ranges for measurements to filter unwanted objects

• Group objects in classes based on measurements

• Resolve clustered objects with Watershed, Auto-Split, and Cluster analysis tools

• Use colors to separate and measure objects in color images

• Display measurements as histograms and scattergrams

• Manually tag, count, and classify objects

• Work with Auto Threshold

• Includes option to manually tag, count, and classify objects

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Object Tracking
 

• Tracking tools allow you to follow objects in a sequence image and measure distance traveled manually, automatically, and semi-automatically

• Correctional tracking allows you to track objects when image segmentation is difficult or not possible

• Static & Dynamic Intensity tracking allows you to track and graph the changing of intensity parameters over time within an area of interest
   

Spatial Measurements
 

• Snap feature allows you to automatically save measurments into images for documentation

• Scale bar for X and Y measurements can be placed on your image as an overlay

• Measure co-localization of probes in seperate gray scale images or within a color image

• Work with weighted centroid, clumpiness, heterogeneity, min/max density, dendrite length, end point, fractal dimension, margination, and additional perimeter measurments

• Record Pass/Fail routines in a macro and play back when needed

• Measure best-fit line, arc, and circle with metrology tools

• Outline objects automatically with Auto-Trace feature

• Measure lengths, areas, perimeters, and angles

• Calculate max, min, and average thickness between lines

• Use the Caliper Tool for edge detection and measurement
   

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Image Analysis
 

• Visualize image data with scattergrams, histograms, and line profiles

• Measurement equation editor - New in 6.0

• Export data via DDE to Origin® or Microsoft® Excel - New in 6.0

• Dynamically link to overlays in an image - New in 6.0

• Color management tools to manage inputs and outputs for your entire imaging system to achieve true color fidelity - New in 6.0

• Display "Dark Mode" for low light laboratory environments - New in 6.0

• Display image intensity values in a 3D Plot with Surface Plot

• Collect data from multiple images with Data Collector

• Live data collecting and charting - New in 6.0

• Calculate straight line, circle, irregular line, or area histograms

• Use new thickness measurement options

• Analyze percent area of multiple threshold levels

• Define and manage multiple areas of interest (AOI) in a single image

• Analyze RGB, HSI, HSV, or YIQ content of color images

• Combine image with background correction for precise intensity or optical density measurements

• Display background corrected line profiles

• Output intensity map in ASCII format

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Image Annotation
 

• Work with non-destructive image annotation

• Add text, simple graphics, and arrows

• Modify your palette or colors

Image Database
 

• Use built-in database or archive, manage, and extract knowledge from your images with the complimentary 6-month trial IQbase 2.0 Image Database, included with Image-Pro Plus Version 6.0 - New in 6.0

• Locate images through keyword searches or view in a thumbnail gallery

• Print full-size or image galleries from inside the database

• Batch process multiple discreet images

• Perform SQL multi-parameter searches

Report Generator
 

• Create customized reports with images, data, and text

• Create and save report templates

Printing and Publishing
 

• Print frames from a sequence or montage of single images with the Mosaic printing feature

• Output gray scale and color images to Windows® printers

• Maintain complete control over image position on your page

• Print tiles to make poster size prints

• Use the Test Strip feature for selection of best output

• Screen capture individual images or entire screen

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Internet Support
 

• Send e-mail messages within Image-Pro Plus

• Review, send, and receive data over the Internet using IRC (Internet Relay Chat)

Customization & Macro Programming 
 

• Floating workflow toolbar builder allows you to create unique toolbars for your specific work process

• Built-in configurations customize Image-Pro Plus for your industry needs, life science or industrial

• Customize your application environment with the interactive menu editor - New in 6.0

• Demo macro browser includes updated running examples

• Use expanded example code in your own macros

• Use the IPBASIC engine with debugger, full editor, and dialog builder

• Record sessions and play them back in Basic Syntax

• Assign scripts to files and function keys

• Add basic loops and conditionals

• Macro editor upgrades, including command recognition - New in 6.0

• Use with COM objects such as Microsoft® Word or Excel

• Integrate and customize macros with Microsoft® Visual Basic® or Visual C++®

• Work with the Dialog Box Editor

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Image Data and File Format Support
 

• Read TIFF, IPW, JPEG, JPEG2000, EXIF JPEG Flat (binary), TGA, BMP, PhotoCD, PICT, CUT, PCX, GEL, PCT, and HDF

• Write and convert files to TIFF, IPW, JPEG, JPEG2000, Flat, TGA, BMP, PICT, PCX, and EPS

• Stack and Confocal read file support for SEQ (Image-Pro Sequence), STK (Metamorph Stack), PIC (Biorad confocal), LSM (Zeiss confocal), DEB and AVZ (Autoquant Stack), LEI (Leica Confocal), QED, Fluoview 1000 and DM3 (Digital Micrograph)

• Read and write SEQ and AVI files

• Support for 24-, 36-, and 48-bit color; 8-, 12-, and 16-bit gray scale as well as 32 bit floating point images

• JPEG, LZW, and RLE compression supported

• Batch convert files

• Output data to ASCII, WK1, or XLS for input for spreadsheets

• Transfer images, data graphs, and data files via DDE or Clipboard

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