Image Pro Plus Features

Memory Management

Memory Management feature allows you to load and analyze image sets that are larger than your available RAM memory

Image Processing Tools

Stitch and tile images
Automatic alignment of image stacks to correct for stereo, rotational, and other types of shifts
N-dimensional viewer lets you choose variables to display from image sets
Create in-focus composite images from partially in-focus source image stacks with EDF (Extended Depth of Field)
EDF Test Strips automatically tests all EDF options on your images with a single click
Set Manager allows you to easily organize, extract, and navigate multiple image sets
Dye Manager lets you apply dyes to gray scale images by choosing emission wavelength
Local Zoom tool allows you to simultaneously view your entire image in one window, while viewing a more magnified area of that image in a second window
Work with topographic maps for composite in-focus images
Create composite in-focus images with maximum local contrast, maximum through depth contrast, maximum intensity and minimum intensity
Create composite images from an infinite number of source gray scale images with the Color Composite Tool
Use the Sequence Gallery to produce a montage image that allows for preview of all image analysis processes on image stacks
Perform spatial and logical arithmetic, as well as image alignment operations
Combine images with Image Overlay
Create, print, or save a collage of several images with Mosaic Image
Combine the results of two segmentation algorithms with Restricted Dilation Feature
Create grids for intercept counting
Use Image Registration tools for comparative analysis

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Image Enhancement Tools

Edit image intensity level with Display Range
Work with background subtraction and correction
Manually or automatically enhance color and contrast using equalization, gamma correction, contouring, or thresholding
Invert, reset, and apply contrast to images
Reverse pixel values with automatic image inversion

Image Filtering

Work with Filter dialogs that include descriptions and interactive preview windows
Use Enhancement filters that include Low-Pass, Hi-Pass, Gaussian, Hi-Gaussian, Sharpen, Flatten, Median, Rank, Local Equalization, and Despeckle in user-definable kernel sizes
Outline objects with various Edge Filter methods including Sobel, Roberts, Laplace, Variance, Phase, Horizontal, and Vertical
Process objects with Morphological filters including Erode, Dilate, Open, Close, Top Hat, Well, Branch/End, Watershed, Thinning, Pruning, Distance, and Reduce in user-definable kernel sizes
Work with Large Spectral Filters: Fast-acting, large kernel size (up to 4000 x 4000) filters that include Low-Pass, Hi-Pass, Band-Pass, and Edge filters
Apply filters to individual frames or to entire sequence
Customize kernel files for morphological and convolution filters

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Fast Fourier Transform (FFT)

Create forward and inverse transforms
Edit the spectrum directly including Low-Pass, High-Pass, unsharpen, spike cut, and spike boost
Save and reload data

Fluorescense Lookup Tables and Overlays

New feature to apply tint to gray scale images automatically
Extract and merge multiple color channels (including RGB, HSI, HSV, and YIQ) simultaneously
Use color information to separate objects from background
Segment image based on the color channel histogram or color cube
Set pre-defined and custom color spreads (ranges) with the Pseudocolor tool

3D Imaging Tools (Requires Open GL graphics card)

Voxel-based 3D filters for use with 3D volumetric, time-lapse and other 3D image sets - New in 6.0
Built-in Open GL viewer for interactive 3D volume rendering - New in 6.0

SharpStack Deconvolution Tools

Built-in real-time 2D deconvolution - New in 6.2
Built-in Nearest Neighbor and No-Neighbor deconvolution - New in 6.2

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Spatial and Intensity Calibration

Calibration wizard helps you to calibrate an active image and create reference and system calibrations
Use X and Y scale bars as a nondestructive overlay
Work with intensity or spatial measurements
Create and display spatial calibration markers
Create and display system calibrations
Use pre-defined spatial calibration units
Save and recall all calibrations
Calculate derived calibrations automatically
Store information about your microscope in lens information files

Classify Objects

Count and Size objects automatically
Measure areas, perimeters, lengths, roundness, major and minor axes, aspects, angles, centroids, holes, population density - over 50 measurements
Identify over 100,000 objects per frame (memory dependent) - New in 6.0
Set ranges for measurements to filter unwanted objects
Group objects in classes based on measurements
Resolve clustered objects with Watershed, Auto-Split, and Cluster analysis tools
Use colors to separate and measure objects in color images
Display measurements as histograms and scattergrams
Manually tag, count, and classify objects
Work with Auto Threshold
Includes option to manually tag, count, and classify objects

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Object Tracking

Tracking tools allow you to follow objects in a sequence image and measure distance traveled manually, automatically, and semi-automatically
Correctional tracking allows you to track objects when image segmentation is difficult or not possible
Static & Dynamic Intensity tracking allows you to track and graph the changing of intensity parameters over time within an area of interest

Spatial Measurements

Snap feature allows you to automatically save measurments into images for documentation
Scale bar for X and Y measurements can be placed on your image as an overlay
Measure co-localization of probes in seperate gray scale images or within a color image
Work with weighted centroid, clumpiness, heterogeneity, min/max density, dendrite length, end point, fractal dimension, margination, and additional perimeter measurments
Record Pass/Fail routines in a macro and play back when needed
Measure best-fit line, arc, and circle with metrology tools
Outline objects automatically with Auto-Trace feature
Measure lengths, areas, perimeters, and angles
Calculate max, min, and average thickness between lines
Use the Caliper Tool for edge detection and measurement

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Image Analysis

Visualize image data with scattergrams, histograms, and line profiles
Measurement equation editor - New in 6.0
Export data via DDE to Origin® or Microsoft® Excel - New in 6.0
Dynamically link to overlays in an image - New in 6.0
Color management tools to manage inputs and outputs for your entire imaging system to achieve true color fidelity - New in 6.0
Display "Dark Mode" for low light laboratory environments - New in 6.0
Display image intensity values in a 3D Plot with Surface Plot
Collect data from multiple images with Data Collector
Live data collecting and charting - New in 6.0
Calculate straight line, circle, irregular line, or area histograms
Use new thickness measurement options
Analyze percent area of multiple threshold levels
Define and manage multiple areas of interest (AOI) in a single image
Analyze RGB, HSI, HSV, or YIQ content of color images
Combine image with background correction for precise intensity or optical density measurements
Display background corrected line profiles
Output intensity map in ASCII format

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Image Annotation

Work with non-destructive image annotation
Add text, simple graphics, and arrows
Modify your palette or colors

Image Database

Use built-in database or archive, manage, and extract knowledge from your images with the complimentary 6-month trial
IQbase 2.0 Image Database, included with Image-Pro Plus Version 6.0 - New in 6.0
Locate images through keyword searches or view in a thumbnail gallery
Print full-size or image galleries from inside the database
Batch process multiple discreet images
Perform SQL multi-parameter searches

Report Generator

Create customized reports with images, data, and text
Create and save report templates

Printing and Publishing

Print frames from a sequence or montage of single images with the Mosaic printing feature
Output gray scale and color images to Windows® printers
Maintain complete control over image position on your page
Print tiles to make poster size prints
Use the Test Strip feature for selection of best output
Screen capture individual images or entire screen

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Internet Support

Send e-mail messages within Image-Pro Plus
Review, send, and receive data over the Internet using IRC (Internet Relay Chat)

Customization & Macro Programming

Floating workflow toolbar builder allows you to create unique toolbars for your specific work process
Built-in configurations customize Image-Pro Plus for your industry needs, life science or industrial
Customize your application environment with the interactive menu editor - New in 6.0
Demo macro browser includes updated running examples
Use expanded example code in your own macros
Use the IPBASIC engine with debugger, full editor, and dialog builder
Record sessions and play them back in Basic Syntax
Assign scripts to files and function keys
Add basic loops and conditionals
Macro editor upgrades, including command recognition - New in 6.0
Use with COM objects such as Microsoft® Word or Excel
Integrate and customize macros with Microsoft® Visual Basic® or Visual C++®
Work with the Dialog Box Editor

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Image Data and File Format Support

Read TIFF, IPW, JPEG, JPEG2000, EXIF JPEG Flat (binary), TGA, BMP, PhotoCD, PICT, CUT, PCX, GEL, PCT, and HDF
Write and convert files to TIFF, IPW, JPEG, JPEG2000, Flat, TGA, BMP, PICT, PCX, and EPS
Stack and Confocal read file support for SEQ (Image-Pro Sequence), STK (Metamorph Stack), PIC (Biorad confocal),
LSM (Zeiss confocal), DEB and AVZ (Autoquant Stack), LEI (Leica Confocal), QED, Fluoview 1000 and DM3 (Digital Micrograph)
Read and write SEQ and AVI files
Support for 24-, 36-, and 48-bit color; 8-, 12-, and 16-bit gray scale as well as 32 bit floating point images
JPEG, LZW, and RLE compression supported
Batch convert files
Output data to ASCII, WK1, or XLS for input for spreadsheets
Transfer images, data graphs, and data files via DDE or Clipboard